1. If a cytometer were available on an AUV, how would you use it?

     - what science question would you address? What paper would you write?

     - what observational challenges do you face?

     - coastal zones vs. open ocean, eddies, midwater vs. surface

2.  Range of target organisms in mixed populations?

     - size range

     - concentration range

     - methods of discrimination (optical, fluorescent properties, etc)

3. Which features are more or less important?

     - value of imaging (on-demand, occasional, primary)

     - value of physical sorting, pick-and-accumulate

     - real time or post processing

     - fluorescent labeling

     - traditional gating vs. computational population ID (how to find rare and hidden populations)

4. Value of new features of integration on autonomous mobile platform?

     - adaptive behavior, contextual sensors data

     - real-time data for reconnaissance and surveying  

     - persistent presence, high-res

5. Any new trends in cytometry, alternative approaches, etc or you aware of?